We use and offer various techniques for metabolome analysis including targeted metabolomics, untargeted metabolomics and flux analyses of biological samples.

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Targeted Metabolomics

Targeted metabolomics is a powerful analytical approach focused on the precise measurement of predefined metabolites within biological systems. By using highly sensitive techniques such as triple quadrupole mass spectrometers, it enables accurate quantification of specific compounds involved in key metabolic pathways. This method is widely applied in clinical research, drug development, and biomarker discovery, offering deep insights into physiological states, disease mechanisms, and therapeutic responses.

For targeted metabolomics, we select the most suitable analytical platform based on the specific metabolites of interest. Our capabilities include LC-QTRAP 5500 and LC-TripleTOF 5600+ systems (both Agilent LC and SCIEX MS), as well as GC-MS instruments, both single and triple quadrupole (both Agilent Technologies). This flexible setup ensures optimal sensitivity and accuracy for a wide range of analytes.

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Untargeted Metabolomics

Untargeted metabolomics provides a comprehensive, discovery-driven analysis within a biological sample, capturing a broad spectrum of metabolites without prior selection. Using advanced high-resolution techniques, this approach enables the detection and relative quantification of hundreds to thousands of compounds in a single run. It is particularly valuable for uncovering novel biomarkers, identifying unexpected metabolic changes, and generating new hypotheses about biological processes. By offering a global view of the metabolome, untargeted metabolomics complements targeted analyses and plays a key role in systems biology, disease research, and precision medicine.

For untargeted metabolomics, we employ an HPLC–Orbitrap Exploris 480 system (Thermo Fisher Scientific) to enable high-resolution, discovery-driven analysis. To maximize metabolome coverage, we use a multimodal workflow in which each sample is analyzed across complementary LC–MS conditions, including reverse-phase chromatography (RP) for non-polar compounds and hydrophilic interaction liquid chromatography (HILIC) for polar metabolites, each measured in both positive and negative ionization modes. Data preprocessing is performed using Compound Discoverer (Thermo Fisher Scientific) or MS-DIAL, followed by advanced biostatistical evaluation with our in-house developed R-based analysis pipeline.

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Flux analyses

Flux analysis provides dynamic insight into metabolic activity by tracking the flow of metabolites through biochemical pathways over time. Typically performed using stable isotope labeling (e.g., ¹³C or ¹⁵N), this approach reveals how nutrients are processed and how metabolic networks respond to genetic or environmental changes. By going beyond static concentration measurements, flux analysis enables a deeper understanding of pathway utilization, cellular function, and metabolic regulation, making it a valuable tool in areas such as systems biology, biotechnology, and disease research.